Ambergen

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Figure 4: Isolation of PKCα via PC-SNAG and Subsequent Functional Assay: Phorbol Ester Mediated PKCα Translocation in HeLa cells

Figure 4: Isolation of PKCα via PC-SNAG and Subsequent Functional Assay: Phorbol Ester Mediated PKCα Translocation in HeLa cells

HeLa cells were stimulated with 200 nM PMA for 5 min and detergent fractionated into the cytosol and membrane compartments. The graph shows subcellular distribution of PKCα based on kinase activity of the PC-Antibody purified protein.

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