Revolutionizing Proteomics
Rapidly performing global proteomic screens is an important goal in the post-genomic era. AmberGen has developed a patented method (Bead-AMS™) combining matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) with fluorescent imaging of bead-arrays. This method enables rapid screening of a large bead-protein library for interactions with a variety of prey molecules including drugs, serum antibodies/autoantibodies or proteomic digests such as cell lysates:
Bead-AMS™ Proteomic Bead-Array Papers
Proteome-wide drug screening using mass spectrometric imaging of bead-arrays.
Zhou Y, Liu Z, Rothschild KJ, Lim MJ.
Sci Rep. 2016 May 19;6:26125. doi: 10.1038/srep26125.
PMID:27194112 Free PMC Article
Correlated matrix-assisted laser desorption/ionization mass spectrometry and fluorescent imaging of photocleavable peptide-coded random bead-arrays.
Lim MJ, Liu Z, Braunschweiger KI, Awad A, Rothschild KJ.
Rapid Commun Mass Spectrom. 2014 Jan 15;28(1):49-62. doi: 10.1002/rcm.6754.
PMID:24285390 Free PMC Article
Photocleavage-based affinity purification and printing of cell-free expressed proteins: application to proteome microarrays.
Lim M, Rothschild KJ.
Anal Biochem. 2008 Dec 1;383(1):103-15. doi: 10.1016/j.ab.2008.07.038. Epub 2008 Aug 12.
PMID: 18762158 Free PMC Article
